Description
Real-time PCR System YR06248 – YR06249 leverages innovative signal gathering and spectrum separation technology combined with unique temperature control advancements. This enables it to achieve an unparalleled level of detection sensitivity, effective multi-color crosstalk correction, and precision in temperature uniformity and accuracy. Offered in both 4-channel and 5-channel configurations, this instrument supports a wide array of qPCR dye-based and probe-based chemistries, making it ideal for multiplex PCR applications.
Market Price
The price of high-quality real-time PCR systems like the YR06248 – YR06249 often ranges from $22,500 to $23,800 in the market, depending on various configurations and capabilities. For an accurate and competitive quote, we invite you to request one directly or generate a quote quickly via the Kalstein Plus platform.
Frequently Asked Questions
- What is the difference between the 4-channel and 5-channel configurations? The main difference lies in the number of detection channels. The 5-channel configuration allows for additional multiplexing capabilities.
- Is this system compatible with all qPCR reagent kits? The system is compatible with a wide range of qPCR dye-based and probe-based chemistries.
- How can I get technical support for this product? Our team provides comprehensive support. Please contact us through Kalstein Plus for any assistance.
Advantages and Disadvantages
The YR06248 – YR06249 boasts advanced thermal cycling precision and innovative optic designs that ensure excellent reproducibility and resolution. Its high dynamic range and sensitivity make it ideal for diverse PCR applications. However, its advanced configurations might represent a higher initial investment and require specialized handling and setup.
Product Use in the Field
This system is widely applied in various research and clinical settings, especially in genomics and gene expression analysis. Its precise thermal cycling and versatile chemistry support make it invaluable for rapid, real-time PCR analysis, from absolute quantification to advanced melting curve analysis.
Recommendations
To maximize the lifespan and performance of your YR06248 – YR06249, ensure regular maintenance and calibration. Follow the manufacturer’s guidelines carefully and seek professional training for new users. Take advantage of our online resources and support via Kalstein Plus.
Features
- Fast and Precise Thermal Cycling
- Innovative Optic Design
- Excellent Reproducibility
- Up to 10-log dynamic range
- Melting Curve Analysis
- High Resolution
Technical Specifications
| Model | YR06248 | YR06249 |
| Sample capacity | 8*0.1mL PCR strip*12 96*0.1mL individual tube 0.1mL PCR Plate |
|
| Sample volume | 10-50 μL | |
| Heating and Cooling method | Peltier | |
| Maximum ramping rate | 6.0° C/s | |
| Temperature range | 4-100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
| Optical Detecting System | ||
| Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | |
| Detection method | Time-resolved real-time scanning | |
| Excitation/Emission wavelengths | 455-650nm | 510-750nm |
| Detection Channels | 4 (optional 5 channels） | |
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ，（Optional- Cy5.5 for 5-channel model） | |
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
| Sensitivity | 1 copy gene | |
| Resolution | 1.33-fold copies difference in single-plex reaction | |
| Dynamic range | 10 orders of magnitude | |
